Microwave Processing in Diagnostic Electron Microscopy

Diagnostic Transmission Electron Microscopy

The high resolution and sensitivity of electron microscopy is still a valuable tool, and for some diseases it is the gold standard in pathological diagnosis. Today the sample turnaround time for processing in the lab can be significantly reduced from days to hours by the microwave technology. In urgent clinical cases, microwaveassisted tissue processing, in combination with digital image acquis...

Quality standards for diagnostic electron microscopy.

Stirling JW, Woods A. Transmission electron microscopy: diagnostic applications. In: Bancroft JD, Gamble M, eds. Theory and Practice of Histological Techniques, 5th ed. London: Churchill Livingstone; 2002:701-728. JOHN W STIRLING Principal Medical Scientist, Electron Microscope Unit, Anatomical Pathology, Flinders Medical Centre, Bedford Park SA 5042 Australia and Co-Convener RCPA QAP EM progra...

Practical aspects of diagnostic electron microscopy.

The electron microscope technique of negative staining was first used to obtain fundamental information about virus morphology, but in recent years it has developed into a practical method for virus diagnosis. The methods employed are both simple and rapid. The following review discusses in detail the steps that must be taken to obtain good electron microscope preparations and illustrates some ...

Microwave irradiation for shortening the processing time of samples of flagellated bacteria for scanning electron microscopy.

Microwave irradiation (MWI) has been applied to the development of rapid methods to process biological samples for scanning electron microscopy (SEM). In this paper we propose two simple and quick techniques for processing bacteria (Proteus mirabilis and Vibrio mimicus) for SEM using MWI. In the simplest methodology, the bacteria were placed on a cover-glass, air-dried, and submitted to conduct...

Electron Microscopy of Fowl Pox Virus